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Ubiquitylation‐dependent downregulation of Nck regulates its functional activity
Author(s) -
Joseph Noah,
Reicher Barak,
David Ahuvit,
Matalon Omri,
Barda-Saad Mira
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.08.033
Subject(s) - microbiology and biotechnology , effector , downregulation and upregulation , signal transducing adaptor protein , ubiquitin , chemistry , t cell receptor , signal transduction , biology , biochemistry , t cell , gene , immunology , immune system
The Nck adapter protein is involved in key cellular functions, such as actin polymerization and reorganization, serving as a molecular bridge between the surface complex essential for foreign antigen recognition, the T‐cell antigen receptor (TCR), and the actin machinery. However, the mechanisms regulating Nck expression and functions are unknown. In this study, we revealed Nck negative regulation and demonstrated that Nck is ubiquitylated following cellular activation. We identified the molecular determinants and mediators involved in this process. Our data suggest that Nck ubiquitylation might serve as a mechanism controlling Nck‐mediated effector functions during cellular activation.