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Cellulosomal carbohydrate‐binding module from Clostridium josui binds to crystalline and non‐crystalline cellulose, and soluble polysaccharides
Author(s) -
Ichikawa Shunsuke,
Karita Shuichi,
Kondo Makoto,
Goto Masakazu
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.08.032
Subject(s) - cellulosome , cellulose , carbohydrate binding module , polysaccharide , chemistry , carbohydrate , biochemistry , clostridium thermocellum , binding site , cellulase
To understand the lignocellulose degradation activity of the Clostridium josui cellulosome, a carbohydrate‐binding module of the scaffoldin Cj CBM3 was characterized. Cj CBM3 shows binding to crystalline cellulose, non‐crystalline cellulose and soluble polysaccharides. The binding isotherm of Cj CBM3 to acid‐swollen cellulose is best fitted by the Langmuir two‐site model, suggesting that there are two Cj CBM3 binding sites on acid‐swollen cellulose with different affinities. The second site shows lower affinity and larger binding capacity, suggesting that the cellulosome is directly targeted to the cellulose surface with high affinity, where larger amounts of the cellulosome bind to cellulose with low affinity.