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Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development
Author(s) -
Zhang Youjun,
Sun Feng,
Fettke Joerg,
Schöttler Mark Aurel,
Ramsden Lawrence,
Fernie Alisdair R.,
Lim Boon Leong
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.08.019
Subject(s) - sucrose , sucrose phosphate synthase , photosynthesis , fructose , phloem , sucrose synthase , invertase , starch , heterologous expression , sink (geography) , industrial crop , carbohydrate metabolism , biology , botany , horticulture , chemistry , biochemistry , solanaceae , gene , cartography , geography , recombinant dna
Changes in carbon flow and sink/source activities can affect floral, architectural, and reproductive traits of plants. In potato, overexpression (OE) of the purple acid phosphatase 2 of Arabidopsis ( AtPAP2 ) resulted in earlier flowering, faster growth rate, increased tubers and tuber starch content, and higher photosynthesis rate. There was a significant change in sucrose, glucose and fructose levels in leaves, phloem and sink biomass of the OE lines, consistent with an increased expression of sucrose transporter 1 (StSUT1). Furthermore, the expression levels and enzyme activity of sucrose‐phosphate synthase (SPS) were also significantly increased in the OE lines. These findings strongly suggest that higher carbon supply from the source and improved sink strength can improve potato tuber yield.