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Paired related homeobox protein‐like 1 (Prrxl1) controls its own expression by a transcriptional autorepression mechanism
Author(s) -
Monteiro César B.,
Costa Mariana F.,
Reguenga Carlos,
Lima Deolinda,
Castro Diogo S.,
Monteiro Filipe A.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.08.006
Subject(s) - homeobox , chromatin immunoprecipitation , homeobox a1 , emx2 , homeobox protein nkx 2.5 , cdx2 , biology , transcription factor , psychological repression , microbiology and biotechnology , immunoprecipitation , spinal cord , dlx5 , gene , genetics , gene expression , promoter , neuroscience
The homeodomain factor paired related homeobox protein‐like 1 (Prrxl1) is crucial for proper assembly of dorsal root ganglia (DRG)–dorsal spinal cord (SC) pain‐sensing circuit. By performing chromatin immunoprecipitation with either embryonic DRG or dorsal SC, we identified two evolutionarily conserved regions (i.e. proximal promoter and intron 4) of Prrxl1 locus that show tissue‐specific binding of Prrxl1. Transcriptional assays confirm the identified regions can mediate repression by Prrxl1, while gain‐of‐function studies in Prrxl1 expressing ND7/23 cells indicate Prrxl1 can down‐regulate its own expression. Altogether, our results suggest that Prrxl1 uses distinct regulatory regions to repress its own expression in DRG and dorsal SC.