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Ribonomic approaches to study the RNA‐binding proteome
Author(s) -
Faoro Camilla,
Ataide Sandro F.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.07.039
Subject(s) - rna binding protein , rna , ribonucleoprotein , computational biology , proteome , biology , gene expression , non coding rna , microbiology and biotechnology , gene , chemistry , genetics
Gene expression is controlled through a complex interplay among mRNAs, non‐coding RNAs and RNA‐binding proteins (RBPs), which all assemble along with other RNA‐associated factors in dynamic and functional ribonucleoprotein complexes (RNPs). To date, our understanding of RBPs is largely limited to proteins with known or predicted RNA‐binding domains. However, various methods have been recently developed to capture an RNA of interest and comprehensively identify its associated RBPs. In this review, we discuss the RNA‐affinity purification methods followed by mass spectrometry analysis (AP‐MS); RBP screening within protein libraries and computational methods that can be used to study the RNA‐binding proteome (RBPome).