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The transcription factors Sox5 and Sox9 regulate Catsper1 gene expression
Author(s) -
Mata-Rocha Minerva,
Hernández-Sánchez Javier,
Guarneros Gabriel,
de la Chesnaye Elsa,
Sánchez-Tusié Ana A.,
Treviño Claudia L.,
Felix Ricardo,
Oviedo Norma
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.07.024
Subject(s) - transactivation , chromatin immunoprecipitation , biology , transcription factor , transcription (linguistics) , chromatin , promoter , gene , genetics , microbiology and biotechnology , gene expression , linguistics , philosophy
Catsper is a Ca 2+ permeable channel required for sperm hyperactivation. In spite of its central role in male fertility, the transcriptional mechanisms that regulate Catsper1 expression are ill defined. In this work, we describe the identification and characterization of important regulatory elements in the murine Catsper1 gene proximal promoter. Four transcription start sites and three functional Sox‐binding sites were identified in the Catsper1 promoter. Interestingly, transcription factors Sox5 and Sox9 caused a significant increase in transactivation of the Catsper1 promoter in heterologous systems, and chromatin immunoprecipitation assays showed that both transcription factors interact with the Catsper1 promoter in vivo. These results provide new insights into the molecular mechanisms that control Catsper channel expression.