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Hypoxia‐induced miR‐497 decreases glioma cell sensitivity to TMZ by inhibiting apoptosis
Author(s) -
Lan Jin,
Xue Yajun,
Chen Huairui,
Zhao Sanhu,
Wu Zhijian,
Fang Jun,
Han Cong,
Lou Meiqing
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.07.021
Subject(s) - glioma , apoptosis , cancer research , ectopic expression , hypoxia (environmental) , chemotherapy , suppressor , microrna , biology , chemistry , cell culture , gene , genetics , organic chemistry , oxygen
Understanding the resistance of glioma cells to chemotherapy has been an enormous challenge. In particular, mechanisms by which tumor cells acquire resistance to chemotherapy under hypoxic conditions are not fully understood. In this study, we have found that miR‐497 is overexpressed in glioma and that hypoxia can induce the expression of miR‐497 at the transcriptional level by binding with the hypoxia response element in the promoter. Ectopic overexpression of miR‐497 promotes chemotherapy resistance in glioma cells by targeting PDCD4, a tumor suppressor that is involved in apoptosis. In contrast, the inhibition of miR‐497 enhances apoptosis and increases the sensitivity of glioma cells to TMZ. These results suggest that miR‐497 is a potential molecular target for glioma therapy.