Premium
Dynein‐dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles
Author(s) -
Silva Patrícia M.A.,
Reis Rita M.,
Bolanos-Garcia Victor M.,
Florindo Claudia,
Tavares Álvaro A.,
Bousbaa Hassan
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.07.011
Subject(s) - kinetochore , dynein , bub1 , spindle checkpoint , microbiology and biotechnology , mad2 , spindle apparatus , dynactin , microtubule , spindle pole body , biology , chemistry , cell division , genetics , gene , cell , chromosome
A predominant mechanism of spindle assembly checkpoint (SAC) silencing is dynein‐mediated transport of certain kinetochore proteins along microtubules. There are still conflicting data as to which SAC proteins are dynein cargoes. Using two ATP reduction assays, we found that the core SAC proteins Mad1, Mad2, Bub1, BubR1, and Bub3 redistributed from attached kinetochores to spindle poles, in a dynein‐dependent manner. This redistribution still occurred in metaphase‐arrested cells, at a time when the SAC should be satisfied and silenced. Unexpectedly, we found that a pool of Hec1 and Mis12 also relocalizes to spindle poles, suggesting KMN components as additional dynein cargoes. The potential significance of these results for SAC silencing is discussed.