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Calcium signaling mediates the response to cadmium toxicity in Saccharomyces cerevisiae cells
Author(s) -
Ruta Lavinia L.,
Popa Valentina C.,
Nicolau Ioana,
Danet Andrei F.,
Iordache Virgil,
Neagoe Aurora D.,
Farcasanu Ileana C.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.07.001
Subject(s) - cytosol , saccharomyces cerevisiae , calcium , cadmium , homeostasis , chemistry , yeast , biophysics , biochemistry , toxicity , microbiology and biotechnology , biology , enzyme , organic chemistry
The involvement of Ca 2+ in the response to high Mn 2+ , Co 2+ , Ni 2+ , Cu 2+ , Zn 2+ , Cd 2+ , and Hg 2+ was investigated in Saccharomyces cerevisiae . The yeast cells responded through a sharp increase in cytosolic Ca 2+ when exposed to Cd 2+ , and to a lesser extent to Cu 2+ , but not to Mn 2+ , Co 2+ , Ni 2+ , Zn 2+ , or Hg 2+ . The response to high Cd 2+ depended mainly on external Ca 2+ (transported through the Cch1p/Mid1p channel) but also on vacuolar Ca 2+ (released into the cytosol through the Yvc1p channel). The adaptation to high Cd 2+ was influenced by perturbations in Ca 2+ homeostasis. Thus, the tolerance to Cd 2+ often correlated with sharp Cd 2+ ‐induced cytosolic Ca 2+ pulses, while the Cd 2+ sensitivity was accompanied by the incapacity to rapidly restore the low cytosolic Ca 2+ .