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MicroRNA‐145 directly targets the insulin‐like growth factor receptor I in human bladder cancer cells
Author(s) -
Zhu Zhaowei,
Xu Tianyuan,
Wang Li,
Wang Xianjin,
Zhong Shan,
Xu Chen,
Shen Zhoujun
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.06.059
Subject(s) - microrna , gene knockdown , oncogene , small interfering rna , insulin like growth factor , bladder cancer , cancer research , insulin like growth factor receptor , cancer , growth factor , cancer cell , cell growth , biology , apoptosis , three prime untranslated region , chemistry , receptor , untranslated region , microbiology and biotechnology , cell culture , messenger rna , transfection , cell cycle , gene , biochemistry , genetics
The insulin‐like growth factor receptor I (IGF‐IR) is a proto‐oncogene with potent mitogenic and antiapoptotic activities. It has been reported that expression of IGF‐IR is up‐regulated in bladder cancer. Here, we assessed whether microRNA‐145 (miR‐145) regulates IGF‐IR expression in bladder cancer. In our study, miR‐145 was shown to directly target IGF‐IR 3′‐untranslated region (UTR) in human bladder cancer cells. Small interfering RNA (siRNA)‐ and miR‐145‐mediated IGF‐IR knockdown experiments revealed that miR‐145 promotes cell apoptosis, and suppresses cell proliferation and migration through suppression of IGF‐IR expression. Taken together, our data suggest that miR‐145 may inhibit bladder cancer initiation by affecting IGF‐IR signaling.