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Food entrains clock genes but not metabolic genes in the liver of suprachiasmatic nucleus lesioned rats
Author(s) -
Sabath Elizabeth,
Salgado-Delgado Roberto,
Guerrero-Vargas Natali N.,
Guzman-Ruiz Mara A.,
del Carmen Basualdo Maria,
Escobar Carolina,
Buijs Ruud M.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.06.045
Subject(s) - suprachiasmatic nucleus , clock , biology , endocrinology , medicine , circadian clock , circadian rhythm , gene , peroxisome proliferator activated receptor , microbiology and biotechnology , biochemistry
Hepatic circadian transcription, considered to be driven by the liver clock, is largely influenced by food even uncoupling it from the suprachiasmatic nucleus (SCN). In SCN lesioned rats (SCNx) we determined the influence of a physiological feeding schedule on the entrainment of clock and clock‐controlled (CCG) genes in the liver. We show that clock genes and the CCG Rev‐erbα and peroxisome proliferator‐activated receptor alpha ( PPARα ) in food‐scheduled intact and SCNx have a robust diurnal differential expression persisting after a 24 h fast. However, hepatic nicotinamide phosphoribosyl transferase ( Nampt ) shows time dependent changes that are lost in intact animals under fasting; moreover, it is unresponsive to the nutrient status in SCNx, indicating a poor reliance on liver clock genes and highlighting the relevance of SCN‐derived signals for its metabolic status‐related expression.