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Twinkle, twinkle little star: Photoswitchable fluorophores for super‐resolution imaging
Author(s) -
Chozinski Tyler J.,
Gag Lauren A.,
Vaughan Joshua C.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.06.043
Subject(s) - resolution (logic) , fluorescence , nanotechnology , superresolution , fluorescence lifetime imaging microscopy , focus (optics) , microscopy , optics , materials science , computer science , physics , artificial intelligence , image (mathematics)
Photoswitchable fluorescent probes are key elements of newly developed super‐resolution fluorescence microscopy techniques that enable far‐field interrogation of biological systems with a resolution of 50 nm or better. In contrast to most conventional fluorescence imaging techniques, the performance achievable by most super‐resolution techniques is critically impacted by the photoswitching properties of the fluorophores. Here we review photoswitchable fluorophores for super‐resolution imaging with discussion of the fundamental principles involved, a focus on practical implementation with available tools, and an outlook on future directions.