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Targeting Alp7/TACC to the spindle pole body is essential for mitotic spindle assembly in fission yeast
Author(s) -
Tang Ngang Heok,
Okada Naoyuki,
Fong Chii Shyang,
Arai Kunio,
Sato Masamitsu,
Toda Takashi
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.06.027
Subject(s) - spindle pole body , biology , centrosome , spindle apparatus , mitosis , microbiology and biotechnology , multipolar spindles , microtubule , genetics , cell division , cell cycle , gene , cell
The conserved TACC protein family localises to the centrosome (the spindle pole body, SPB in fungi) and mitotic spindles, thereby playing a crucial role in bipolar spindle assembly. However, it remains elusive how TACC proteins are recruited to the centrosome/SPB. Here, using fission yeast Alp7/TACC, we have determined clustered five amino acid residues within the TACC domain required for SPB localisation. Critically, these sequences are essential for the functions of Alp7, including proper spindle formation and mitotic progression. Moreover, we have identified pericentrin‐like Pcp1 as a loading factor to the mitotic SPB, although Pcp1 is not a sole platform.