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MicroRNAs function as cis‐ and trans‐acting modulators of peripheral circadian clocks
Author(s) -
Shende Vikram R.,
Kim Sam-Moon,
Neuendorff Nichole,
Earnest David J.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.05.058
Subject(s) - per2 , circadian rhythm , extracellular , intracellular , microrna , microbiology and biotechnology , biology , circadian clock , function (biology) , extracellular vesicle , endogeny , clock , microvesicles , gene , neuroscience , genetics , endocrinology
Based on their extracellular expression and targeting of the clock gene Bmal1 , miR‐142‐3p and miR‐494 were analyzed for evidence of vesicle‐mediated communication between cells and intracellular functional activity. Our studies demonstrate that: miR‐142‐3p + miR‐494 overexpression decreases endogenous BMAL1 levels, increases the period of Per2 oscillations, and increases extracellular miR‐142‐3p/miR‐494 abundance in conditioned medium; miRNA‐enriched medium increases intracellular expression of miR‐142‐3p and represses Bmal1 3′‐UTR activity in naïve cells; and inhibitors of vesicular trafficking modulate intercellular communication of these miRNAs and ensemble Per2 rhythms. Thus, miR‐142‐3p and miR‐494 may function as cis‐ and trans‐acting signals contributing to local temporal coordination of cell‐autonomous circadian clocks.