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Characterization of the contractile P2Y14 receptor in mouse coronary and cerebral arteries
Author(s) -
Haanes Kristian Agmund,
Edvinsson Lars
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.05.044
Subject(s) - purinergic receptor , basilar artery , receptor , extracellular , cerebral arteries , medicine , pertussis toxin , coronary arteries , contraction (grammar) , endocrinology , p2 receptor , artery , biology , anatomy , chemistry , microbiology and biotechnology , g protein
Extracellular UDP‐glucose can activate the purinergic P2Y14 receptor. The aim of the present study was to examine the physiological importance of P2Y14 receptors in the vasculature. The data presented herein show that UDP‐glucose causes contraction in mouse coronary and basilar arteries. The EC 50 values and immunohistochemistry illustrated the strongest P2Y14 receptor expression in the basilar artery. In the presence of pertussis toxin, UDP‐glucose inhibited contraction in coronary arteries and in the basilar artery it surprisingly caused relaxation. After organ culture of the coronary artery, the EC 50 value decreased and an increased staining for the P2Y14 receptor was observed, showing receptor plasticity.