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Synaptotagmin 11 interacts with components of the RNA‐induced silencing complex RISC in clonal pancreatic β‐cells
Author(s) -
Milochau Alexandra,
Lagrée Valérie,
Benassy Marie-Noëlle,
Chaignepain Stéphane,
Papin Julien,
Garcia-Arcos Itsaso,
Lajoix Anne,
Monterrat Carole,
Coudert Laetitia,
Schmitter Jean-Marie,
Ochoa Begoña,
Lang Jochen
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.05.031
Subject(s) - synaptotagmin 1 , rna induced silencing complex , microbiology and biotechnology , gene silencing , gene isoform , chemistry , rna , rna interference , biology , golgi apparatus , biochemistry , gene , vesicle , synaptic vesicle , membrane , endoplasmic reticulum
Synaptotagmins are two C 2 domain‐containing transmembrane proteins. The function of calcium‐sensitive members in the regulation of post‐Golgi traffic has been well established whereas little is known about the calcium‐insensitive isoforms constituting half of the protein family. Novel binding partners of synaptotagmin 11 were identified in β‐cells. A number of them had been assigned previously to ER/Golgi derived‐vesicles or linked to RNA synthesis, translation and processing. Whereas the C2A domain interacted with the Q‐SNARE Vti1a, the C2B domain of syt11 interacted with the SND1, Ago2 and FMRP, components of the RNA‐induced silencing complex (RISC). Binding to SND was direct via its N‐terminal tandem repeats. Our data indicate that syt11 may provide a link between gene regulation by microRNAs and membrane traffic.