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Low expression level of OB‐Rb results from constitutive translocational attenuation attributable to a less efficient signal sequence
Author(s) -
Choi Ilho,
Park Joong-Yeol,
Song Youngsup,
Yoon Seung-Yong,
Chang Eun-Ju,
Kang Sang-Wook
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.05.025
Subject(s) - chromosomal translocation , sequence (biology) , signal peptide , signal (programming language) , expression (computer science) , signal transduction , chemistry , microbiology and biotechnology , biology , peptide sequence , gene , biochemistry , computer science , programming language
OB‐Rb is a crucial factor for leptin signaling. This study was initially motivated by the observation that OB‐Rb expression is constitutively inhibited in the early secretory pathway. Our analyses reveal that OB‐Rb contains a less hydrophobic, but functionally active N‐terminal signal sequence. Constitutive translocational attenuation attributable to a less efficient signal sequence proved to be a reason for low protein level of OB‐Rb. By contrast, enhanced signal sequence efficiency rescues translocation and cell surface expression of OB‐Rb, and eventually potentiates leptin signaling. These observations provide considerable insight into the therapeutic enhancement of OB‐Rb translocation as a potential strategy for leptin resistance.