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Downregulation of miR‐638 promotes invasion and proliferation by regulating SOX2 and induces EMT in NSCLC
Author(s) -
Xia Yang,
Wu Yanhu,
Liu Bin,
Wang Pengli,
Chen Yijiang
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.05.002
Subject(s) - gene silencing , downregulation and upregulation , sox2 , microrna , epithelial–mesenchymal transition , cell growth , cancer research , metastasis , lung cancer , chemistry , biology , cancer , microbiology and biotechnology , medicine , gene , transcription factor , biochemistry , genetics
Aberrant expression of microRNAs has been shown to regulate the biological processes of lung cancer cells. However, the role of miR‐638 in the development of NSCLC is still unclear. In this study, low miR‐638 and high SOX2 were shown to be associated with tumor size and metastasis of NSCLC patients. Downregulated miR‐638 could promote cell invasion and proliferation, while high miR‐638 expression reversed the effect. Furthermore, miR‐638 could regulate SOX2 by directly binding to its 3′‐UTR. Silencing of SOX2 by siRNA partially abolished the enhancement of cell invasion and proliferation induced by downregulated miR‐638. Aberrant miR‐638 expression could modulate the expression levels of markers of epithelial‐to‐mesenchymal transition. Our results indicate that miR‐638 may play a pivotal role in the development of NSCLC.