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Huaier suppresses proliferation and induces apoptosis in human pulmonary cancer cells via upregulation of miR‐26b‐5p
Author(s) -
Wu Tangwei,
Chen Weiqun,
Liu Shuiyi,
Lu Hongda,
Wang Hui,
Kong Deyong,
Huang Xiaodong,
Kong Qinzhi,
Ning Yong,
Lu Zhongxin
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.04.044
Subject(s) - downregulation and upregulation , apoptosis , a549 cell , transfection , pulmonary cancer , microrna , cancer research , lung cancer , ezh2 , chemistry , cancer cell , microbiology and biotechnology , cancer , biology , cell culture , medicine , gene , gene expression , biochemistry , genetics
Various studies have reported that Huaier possesses anti‐tumor effects. However, the mechanisms are not completely elucidated. Here, we found 66 differentially expressed miRNAs in Huaier‐treated pulmonary adenocarcinoma A549 cells, with upregulation of miR‐26b‐5p. Transfection of A549 cells with miR‐26b‐5p mimic inhibited proliferation and induced apoptosis, while transfection of Huaier‐treated A549 cells with a miR‐26b‐5p inhibitor reversed the effects of Huaier. EZH2 was verified as the target of miR‐26b‐5p. Thus, our findings indicate that Huaier might suppress proliferation and induce apoptosis in lung cancer cells via a miR‐26b‐5p‐EZH2‐mediated approach, which provides a new perspective for understanding the anti‐tumor effects of Huaier.