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ERp57 modulates mitochondrial calcium uptake through the MCU
Author(s) -
He Jingquan,
Shi Weikang,
Guo Yu,
Chai Zhen
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.04.041
Subject(s) - mitochondrion , uniporter , microbiology and biotechnology , calcium , gene knockdown , endoplasmic reticulum , chemistry , calcium metabolism , inner mitochondrial membrane , calcium signaling , biochemistry , biology , apoptosis , cytosol , signal transduction , organic chemistry , enzyme
ERp57 participates in the regulation of calcium homeostasis. Although ERp57 modulates calcium flux across the plasma membrane and the endoplasmic reticulum membrane, its functions on mitochondria are largely unknown. Here, we found that ERp57 can regulate the expression of the mitochondrial calcium uniporter (MCU) and modulate mitochondrial calcium uptake. In ERp57‐silenced HeLa cells, MCU was downregulated, and the mitochondrial calcium uptake was inhibited, consistent with the effect of MCU knockdown. When MCU was re‐expressed in the ERp57 knockdown cells, mitochondrial calcium uptake was restored. Thus, ERp57 is a potent regulator of mitochondrial calcium homeostasis.