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A cis ‐encoded sRNA controls the expression of fabH2 in Yersinia
Author(s) -
Lu Pei,
Zhang Yong,
Hu Yangbo,
Francis Matthew S.,
Chen Shiyun
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.04.005
Subject(s) - polynucleotide phosphorylase , yersinia pseudotuberculosis , transfer rna , yersinia , purine nucleoside phosphorylase , biology , gene expression , microbiology and biotechnology , gene , chemistry , bacteria , biochemistry , genetics , rna , enzyme , purine , virulence
YsrH is a novel cis ‐encoded sRNA located on the opposite strand to fabH2 , which is essential for fatty acid biosynthesis in bacteria. In this study, YsrH‐mediated regulation of fabH2 expression was investigated in Yersinia pseudotuberculosis . Constitutive and inducible over‐expression of YsrH decreased the mRNA level of fabH2 , while expression of downstream fabD and fabG remained unaffected. Polynucleotide phosphorylase (PNPase) also played an important role in this regulation process by mediating YsrH decay in the exponential phase. Thus, our data defines a cis ‐encoded sRNA that regulates fatty acid synthesis via a regulatory mechanism also involving PNPase.