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Protein kinase Cθ gene expression is oppositely regulated by GCN5 and EBF1 in immature B cells
Author(s) -
Kikuchi Hidehiko,
Nakayama Masami,
Kuribayashi Futoshi,
Imajoh-Ohmi Shinobu,
Nishitoh Hideki,
Takami Yasunari,
Nakayama Tatsuo
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.03.025
Subject(s) - protein kinase c , gene , microbiology and biotechnology , transcription factor , histone , gene expression , biology , chromatin immunoprecipitation , chromatin , transcription (linguistics) , regulation of gene expression , chemistry , kinase , genetics , promoter , linguistics , philosophy
In this study, we revealed that GCN5 and early B cell factor 1 (EBF1) participate in regulation of protein kinase Cθ (PKCθ) gene expression in an opposite manner in immature B cells. GCN5‐deficiency in DT40 caused drastic down‐regulation of transcription of PKCθ. In contrast, EBF1‐deficiency brought about remarkable up‐regulation of that of PKCθ, and re‐expression of EBF1 dramatically suppressed transcription of PKCθ. Chromatin immunoprecipitation assay revealed that GCN5 binds to the 5′‐flanking region of the chicken PKCθ gene and acetylates histone H3, and EBF1 binds to the 5′‐flanking region of the gene surrounding putative EBF1 binding motifs.