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Quantitative investigation of calcimimetic R568 on beta cell adhesion and mechanics using AFM single‐cell force spectroscopy
Author(s) -
Siamantouras Eleftherios,
Hills Claire E.,
Younis Mustafa Y.G.,
Squires Paul E.,
Liu Kuo-Kang
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.02.058
Subject(s) - force spectroscopy , cell adhesion , atomic force microscopy , adhesion , indentation , nanoindentation , biophysics , materials science , elastic modulus , cantilever , cell mechanics , viscoelasticity , chemistry , contact mechanics , cell , analytical chemistry (journal) , composite material , nanotechnology , thermodynamics , chromatography , physics , biochemistry , cytoskeleton , biology , finite element method
In this study we use a novel approach to quantitatively investigate mechanical and interfacial properties of clonal β‐cells using AFM‐Single Cell Force Spectroscopy (SCFS). MIN6 cells were incubated for 48 h with 0.5 mM Ca 2+ ± the calcimimetic R568 (1 μM). AFM‐SCFS adhesion and indentation experiments were performed by using modified tipless cantilevers. Hertz contact model was applied to analyse force–displacement ( F – d ) curves for determining elastic or Young's modulus ( E ). Our results show CaSR‐evoked increases in cell‐to‐cell adhesion parameters and E modulus of single cells, demonstrating that cytomechanics have profound effects on cell adhesion characterization.