Premium
MiRNA let‐7g regulates skeletal myoblast motility via Pinch‐2
Author(s) -
Boudoukha S.,
Rivera Vargas T.,
Dang I.,
Kropp J.,
Cuvellier S.,
Gautreau A.,
Polesskaya A.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.02.057
Subject(s) - motility , microbiology and biotechnology , biology , pinch , gene expression , myocyte , rna , regulation of gene expression , gene , genetics , physics , nuclear physics
Post‐transcriptional regulation of gene expression by RNA‐binding proteins and by small non‐coding RNAs plays an important role in cell biology. Our previous results show that in murine skeletal myoblasts, the expression of Pinch‐2, a focal adhesion remodeling factor that regulates cell motility, is repressed by an RNA‐binding protein IMP‐2/Igf2bp2. We now show that the expression of Pinch‐2 is also regulated by the miRNA let‐7g. Let‐7g and IMP‐2 repress Pinch‐2 expression independently of each other. A knock‐down of let‐7g leads to an increase in Pinch‐2 expression, and to a decrease of cell motility, which can be reversed by a simultaneous knock‐down of Pinch‐2. We conclude that let‐7g controls the motility of mouse myoblasts in cell culture by post‐transcriptionally regulating the expression of Pinch‐2.