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Drosophila melanogaster as a model system to study long‐chain fatty acid amide metabolism
Author(s) -
Jeffries Kristen A.,
Dempsey Daniel R.,
Behari Anita L.,
Anderson Ryan L.,
Merkler David J.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.02.051
Subject(s) - drosophila melanogaster , fatty acid , amide , metabolism , biochemistry , melanogaster , fatty acid metabolism , chemistry , carbon chain , fatty acid amide hydrolase , long chain , biology , gene , organic chemistry , receptor , cannabinoid receptor , agonist , polymer science
Long‐chain fatty acid amides are cell‐signaling lipids identified in mammals and, recently, in invertebrates, as well. Many details regarding fatty acid amide metabolism remain unclear. Herein, we demonstrate that Drosophila melanogaster is an excellent model system for the study long‐chain fatty acid amide metabolism as we have quantified the endogenous levels of N ‐acylglycines, N‐ acyldopamines, N ‐acylethanolamines, and primary fatty acid amides by LC/QTOF‐MS. Growth of D. melanogaster on media supplemented with [1‐ 13 C]‐palmitate lead to a family of 13 C‐palmitate‐labeled fatty acid amides in the fly heads. The [1‐ 13 C]‐palmitate feeding studies provide insight into the biosynthesis of the fatty acid amides.