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Unsaturated lipids protect the integral membrane peptide gramicidin A from singlet oxygen
Author(s) -
Rokitskaya Tatyana I.,
Kotova Elena A.,
Agapov Igor I.,
Moisenovich Mikhail M.,
Antonenko Yuri N.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.02.046
Subject(s) - singlet oxygen , gramicidin , membrane , peptide , chemistry , biophysics , gramicidin s , oxygen , biochemistry , biology , organic chemistry
In contrast to expectations that unsaturated fatty acids contribute to oxidative stress by providing a source of lipid peroxides, we demonstrated the protective effect of double bonds in lipids on oxidative damage to membrane proteins. Photodynamic inactivation of gramicidin channels was decreased in unsaturated lipid compared to saturated lipid bilayers. By estimating photosensitizer (boronated chlorine e 6 amide) binding to the membrane with the current relaxation technique, the decrease in gramicidin photoinactivation was attributed to singlet oxygen scavenging by double bonds in lipids rather than to the reduction in photosensitizer binding. Gramicidin protection by unsaturated lipids was also observed upon induction of oxidative stress with tert‐butyl hydroperoxide.