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Alt a 1 from Alternaria interacts with PR5 thaumatin‐like proteins
Author(s) -
Gómez-Casado Cristina,
Murua-García Amaya,
Garrido-Arandia María,
González-Melendi Pablo,
Sánchez-Monge Rosa,
Barber Domingo,
Pacios Luis F.,
Díaz-Perales Araceli
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.02.044
Subject(s) - alternaria alternata , alternaria , spore , hypha , virulence , microbiology and biotechnology , pathogenicity , inoculation , biology , thaumatin , gene , downregulation and upregulation , chemistry , horticulture , biochemistry
Alt a 1 is a protein found in Alternaria alternata spores related to virulence and pathogenicity and considered to be responsible for chronic asthma in children. We found that spores of Alternaria inoculated on the outer surface of kiwifruits did not develop hyphae. Nevertheless, the expression of Alt a 1 gene was upregulated, and the protein was detected in the pulp where it co‐localized with kiwi PR5. Pull‐down assays demonstrated experimentally that the two proteins interact in such a way that Alt a 1 inhibits the enzymatic activity of PR5. These results are relevant not only for plant defense, but also for human health as patients with chronic asthma could suffer from an allergic reaction when they eat fruit contaminated with Alternaria .