NAD + ‐linked alcohol dehydrogenase 1 regulates methylglyoxal concentration in  Candida albicans | Zendy

Kwak Min-Kyu | Zendy; Ku MyungHee | Zendy; Kang Sa-Ouk | Zendy

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NAD + ‐linked alcohol dehydrogenase 1 regulates methylglyoxal concentration in Candida albicans

Author(s) -

Kwak Min-Kyu,

Ku MyungHee,

Kang Sa-Ouk

Publication year - 2014

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2014.02.042

Subject(s) - methylglyoxal , nad+ kinase , candida albicans , alcohol dehydrogenase , chemistry , biochemistry , alcohol , dehydrogenase , alcohol oxidoreductase , enzyme , microbiology and biotechnology , biology

We purified a fraction that showed NAD + ‐linked methylglyoxal dehydrogenase activity, directly catalyzing methylglyoxal oxidation to pyruvate, which was significantly increased in glutathione‐depleted Candida albicans . It also showed NADH‐linked methylglyoxal‐reducing activity. The fraction was identified as a NAD + ‐linked alcohol dehydrogenase (ADH1) through mass spectrometric analyses. In ADH1 ‐disruptants of both the wild type and glutathione‐depleted cells, the intracellular methylglyoxal concentration increased significantly; defects in growth, differentiation, and virulence were observed; and G2‐phase arrest was induced.

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