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Membrane androgen receptor sensitive Na + /H + exchanger activity in prostate cancer cells
Author(s) -
Chatterjee Soumya,
Schmidt Sebastian,
Pouli Stella,
Honisch Sabina,
Alkahtani Saad,
Stournaras Christos,
Lang Florian
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.02.040
Subject(s) - sgk1 , sodium–hydrogen antiporter , cytosol , chemistry , androgen receptor , kinase , microbiology and biotechnology , endocrinology , medicine , biophysics , prostate cancer , biochemistry , biology , sodium , enzyme , cancer , organic chemistry
Membrane androgen receptors (mAR) are expressed in several tumors. mAR activation by testosterone albumin conjugates (TAC) suppresses tumor growth and migration. mAR signaling involves phosphoinositide‐3‐kinase (PI3K) and Rho‐associated protein kinase (ROCK). PI3K stimulates serum‐ and glucocorticoid‐inducible kinase SGK1, which in turn activates Na + /H + ‐exchangers (NHE). In prostate cancer cells cytosolic pH (pH i ) was determined utilizing 2′,7′‐bis‐(2‐carboxyethyl)‐5‐(and‐6)‐carboxyfluorescein‐fluorescence and NHE‐activity utilizing Na + ‐dependent cytosolic realkalinization following an ammonium pulse. TAC (100 nM) significantly increased pH i and NHE‐activity, effects abrogated by NHE1‐inhibitor cariporide (10 μM), SGK1‐inhibitors EMD638683 (50 μM) and GSK650349 (10 μM) and ROCK‐inhibitors Y‐27632 (10 μM) and fasudil (100 μM). TAC treatment rapidly and significantly increased cell volume and actin polymerization, effects abolished in the presence of cariporide. Thus, mAR‐activation activates cariporide‐sensitive Na + /H + ‐exchangers, an effect requiring SGK1 and ROCK activity.