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C/EBP‐α, involvement of a novel transcription factor in leptin‐induced VCAM‐1 production in mouse chondrocytes
Author(s) -
Kang Xia,
Xie Qing-Yun,
Zhou Jing-Song,
Zhang Bo,
Liao Dong-Fa,
Wu Hong-Hua,
Zheng Wei,
Pan Xian-Ming,
Quan Yi
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.02.032
Subject(s) - leptin , vcam 1 , medicine , endocrinology , chemistry , cell adhesion molecule , transcription factor , microbiology and biotechnology , biology , gene , obesity , icam 1 , biochemistry
Leptin and vascular cell adhesion molecules‐1 (VCAM‐1) are two important mediators in obesity‐related osteoarthritis, while the molecular mechanism linking leptin to VCAM‐1 production is still obscure. Here we show that leptin upregulates VCAM‐1 mRNA and protein levels in a time‐ and dose‐dependent manner. Mechanistically, leptin induces VCAM‐1 promoter activity by increasing the expression of C/EBP‐α and facilitating its binding to a newly identified element in the VCAM‐1 gene. Gain or loss of function studies reveal a regulatory role of C/EBP‐α on VCAM‐1 expression. Finally, elevated plasma leptin level correlates to increased C/EBP‐α and VCAM‐1 production in chondrocytes from obese mice.