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Crystal structure of the RNA demethylase ALKBH5 from zebrafish
Author(s) -
Chen Weizhong,
Zhang Liang,
Zheng Guanqun,
Fu Ye,
Ji Quanjiang,
Liu Fange,
Chen Hao,
He Chuan
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.02.021
Subject(s) - alkb , demethylase , zebrafish , rna , biology , nucleic acid , biochemistry , demethylation , n6 methyladenosine , microbiology and biotechnology , chemistry , dna , methylation , methyltransferase , gene , gene expression , dna methylation , dna repair , histone
ALKBH5, a member of AlkB family proteins, has been reported as a mammalian N 6 ‐methyladenosine (m 6 A) RNA demethylase. Here we report the crystal structure of zebrafish ALKBH5 (fALKBH5) with the resolution of 1.65 Å. Structural superimposition shows that fALKBH5 is comprised of a conserved jelly‐roll motif. However, it possesses a loop that interferes potential binding of a duplex nucleic acid substrate, suggesting an important role in substrate selection. In addition, several active site residues are different between the two known m 6 A RNA demethylases, ALKBH5 and FTO, which may result in their slightly different pathways of m 6 A demethylation.