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Cyclic mononucleotides modulate potassium and calcium flux responses to H 2 O 2 in Arabidopsis roots
Author(s) -
Ordoñez Natalia Maria,
Marondedze Claudius,
Thomas Ludivine,
Pasqualini Stefania,
Shabala Lana,
Shabala Sergey,
Gehring Chris
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.01.062
Subject(s) - arabidopsis , hydrogen peroxide , chemistry , potassium , second messenger system , calcium , efflux , biophysics , biochemistry , mutant , enzyme , biology , gene , organic chemistry
Cyclic mononucleotides are messengers in plant stress responses. Here we show that hydrogen peroxide (H 2 O 2 ) induces rapid net K + ‐efflux and Ca 2+ ‐influx in Arabidopsis roots. Pre‐treatment with either 10 μM cAMP or cGMP for 1 or 24 h does significantly reduce net K + ‐leakage and Ca 2+ ‐influx, and in the case of the K + ‐fluxes, the cell permeant cyclic mononucleotides are more effective. We also examined the effect of 10 μM of the cell permeant 8‐Br‐cGMP on the Arabidopsis microsomal proteome and noted a specific increase in proteins with a role in stress responses and ion transport, suggesting that cGMP is sufficient to directly and/or indirectly induce complex adaptive changes to cellular stresses induced by H 2 O 2 .