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Valencene oxidase CYP706M1 from Alaska cedar ( Callitropsis nootkatensis )
Author(s) -
Cankar Katarina,
van Houwelingen Adèle,
Goedbloed Miriam,
Renirie Rokus,
de Jong René M.,
Bouwmeester Harro,
Bosch Dirk,
Sonke Theo,
Beekwilder Jules
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.01.061
Subject(s) - chemistry , sesquiterpene , cytochrome p450 , enzyme , biochemistry , stereochemistry
(+)‐Nootkatone is a natural sesquiterpene ketone used in grapefruit and citrus flavour compositions. It occurs in small amounts in grapefruit and is a major component of Alaska cedar ( Callitropsis nootkatensis ) heartwood essential oil. Upon co‐expression of candidate cytochrome P450 enzymes from Alaska cedar in yeast with a valencene synthase, a C. nootkatensis valencene oxidase (CnVO) was identified to produce trans ‐nootkatol and (+)‐nootkatone. Formation of (+)‐nootkatone was detected at 144 ± 10 μg/L yeast culture. CnVO belongs to a new subfamily of the CYP706 family of cytochrome P450 oxidases.