The PGRS domain is responsible for translocation of PE_PGRS30 to cell poles while the PE and the C‐terminal domains localize it to the cell wall | Zendy

Chatrath Shweta | Zendy; Gupta Vineet Kumar | Zendy; Garg Lalit C. | Zendy

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The PGRS domain is responsible for translocation of PE_PGRS30 to cell poles while the PE and the C‐terminal domains localize it to the cell wall

Author(s) -

Chatrath Shweta,

Gupta Vineet Kumar,

Garg Lalit C.

Publication year - 2014

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2014.01.059

Subject(s) - mycobacterium smegmatis , cell wall , microbiology and biotechnology , c terminus , mutant , chromosomal translocation , cell , domain (mathematical analysis) , biology , n terminus , biochemistry , chemistry , biophysics , peptide sequence , gene , amino acid , mycobacterium tuberculosis , medicine , tuberculosis , mathematical analysis , mathematics , pathology

PE_PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE_PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE_PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C‐terminal domain present in few PE_PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE_PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE_PGRS30.

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