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The PGRS domain is responsible for translocation of PE_PGRS30 to cell poles while the PE and the C‐terminal domains localize it to the cell wall
Author(s) -
Chatrath Shweta,
Gupta Vineet Kumar,
Garg Lalit C.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.01.059
Subject(s) - mycobacterium smegmatis , cell wall , microbiology and biotechnology , c terminus , mutant , chromosomal translocation , cell , domain (mathematical analysis) , biology , n terminus , biochemistry , chemistry , biophysics , peptide sequence , gene , amino acid , mycobacterium tuberculosis , medicine , tuberculosis , mathematical analysis , mathematics , pathology
PE_PGRS proteins localize in the mycobacterial cell wall and the cell wall localization of PE_PGRS33 has been shown to be attributed to its PE domain. In this study, we expressed deletion mutants of PE_PGRS30 in Mycobacterium smegmatis to characterize the role of its domains in protein localization. It was revealed that, apart from the PE domain, the C‐terminal domain present in few PE_PGRS proteins carries individual cell wall localization signals. Proteinase K sensitivity assay showed that PE_PGRS30 is exposed on the mycobacterial surface through its PGRS domain. PGRS domain was also shown to be responsible for polar localization of PE_PGRS30.