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H3K9 histone methyltransferase G9a‐mediated transcriptional activation of p21
Author(s) -
Oh Si-Taek,
Kim Kee-Beom,
Chae Yun-Cheol,
Kang Joo-Young,
Hahn Yoonsoo,
Seo Sang-Beom
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.01.039
Subject(s) - pcaf , coactivator , microbiology and biotechnology , promoter , epigenetics , chemistry , apoptosis , transcription (linguistics) , cell cycle , transcription factor , cell cycle checkpoint , cancer research , biology , gene , gene expression , biochemistry , linguistics , philosophy
We report that H3K9 HMTase G9a activates transcription of the cell cycle regulatory gene, p21 , in p53 ‐null H1299 cells. Positive regulation of p21 by G9a is independent of its HMTase activity. We demonstrate that G9a upregulates p21 via interaction with PCAF, and provide evidence that the activating complex is recruited to the p21 promoter upon DNA damage‐inducing agent etoposide treatment. Our study suggests that G9a decreases proliferation and cell viability by increasing the level of p21‐mediated apoptosis. Our results suggest that G9a functions as a coactivator for p21 transcription, and directs cells to undergo apoptosis.