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Effects of palmitoylation on the diffusional movement of BK Ca channels in live cells
Author(s) -
Kim Sulgi,
Lee Byoung-Cheol,
Lee A-Ram,
Won Sehoon,
Park Chul-Seung
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.01.014
Subject(s) - palmitoylation , bk channel , chemistry , biophysics , linker , mutant , cytosol , transmembrane protein , cysteine , biochemistry , membrane potential , biology , enzyme , receptor , computer science , gene , operating system
BK Ca channels are palmitoylated at a cluster of cysteine residues within the cytosolic linker connecting the 1st and 2nd transmembrane domains, and this lipid modification affects their surface expression. To verify the effects of palmitoylation on the diffusional dynamics of BK Ca channels, we investigated their lateral movement. Compared to wild‐type channels, the movement of mutant palmitoylation‐deficient channels was much less confined and close to random. The diffusion of the mutant channel was also much faster than that of the wild type. Thus, the lateral movement of BK Ca channels is greatly influenced by palmitoylation.