Premium
A miR‐155‐dependent microRNA hierarchy in dendritic cell maturation and macrophage activation
Author(s) -
Dueck Anne,
Eichner Alexander,
Sixt Michael,
Meister Gunter
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2014.01.009
Subject(s) - microrna , argonaute , mir 155 , microbiology and biotechnology , psychological repression , biology , messenger rna , blot , transcription factor , rna interference , gene expression , gene , rna , genetics
MicroRNAs (miRNAs) are small RNAs that play important regulatory roles in many cellular pathways. MiRNAs associate with members of the Argonaute protein family and bind to partially complementary sequences on mRNAs and induce translational repression or mRNA decay. Using deep sequencing and Northern blotting, we characterized miRNA expression in wild type and miR‐155‐deficient dendritic cells (DCs) and macrophages. Analysis of different stimuli (LPS, LDL, eLDL, oxLDL) reveals a direct influence of miR‐155 on the expression levels of other miRNAs. For example, miR‐455 is negatively regulated in miR‐155‐deficient cells possibly due to inhibition of the transcription factor C/EBPbeta by miR‐155. Based on our comprehensive data sets, we propose a model of hierarchical miRNA expression dominated by miR‐155 in DCs and macrophages.