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Expression of TLE3 by bone marrow stromal cells is regulated by canonical Wnt signaling
Author(s) -
Kokabu Shoichiro,
Sato Tsuyoshi,
Ohte Satoshi,
Enoki Yuichiro,
Okubo Masahiko,
Hayashi Naoki,
Nojima Junya,
Tsukamoto Sho,
Fukushima Yosuke,
Sakata Yasuaki,
Katagiri Takenobu,
Rosen Vicki,
Yoda Tetsuya
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.12.031
Subject(s) - wnt signaling pathway , osteoblast , runx2 , signal transduction , enhancer , stromal cell , medicine , bone marrow , lrp5 , microbiology and biotechnology , endocrinology , transcription factor , biology , genetics , gene , in vitro
Transducing‐like enhancer of split 3 (TLE3), one of the Groucho/TLE family members, targets Runx2 transcription and suppresses osteoblast differentiation in bone marrow stromal cells (BMSCs). Here, we identify Wnt responsive elements of the TLE3 promoter region through comparative genomic and functional analyses and show that expression of TLE3 is increased by Wnt signaling, which is important for osteoblast differentiation. We also demonstrated that TLE3 is able to suppress canonical Wnt signaling in BMSCs. Taken together, our data suggest that induction of TLE3 by Wnt signaling is part of a negative feedback loop active during osteoblast differentiation.