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A simple method for the determination of reduction potentials in heme proteins
Author(s) -
Efimov Igor,
Parkin Gary,
Millett Elizabeth S.,
Glenday Jennifer,
Chan Cheuk K.,
Weedon Holly,
Randhawa Harpreet,
Basran Jaswir,
Raven Emma L.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.12.030
Subject(s) - heme , reduction (mathematics) , chemistry , hemeprotein , simple (philosophy) , biophysics , biochemistry , biology , mathematics , enzyme , philosophy , geometry , epistemology
We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS‐A domains of the regulatory heme proteins human NPAS2 ( E m = −115 mV ± 2 mV, pH 7.0) and human CLOCK ( E m = −111 mV ± 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.