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Proteasome involvement in a complex cascade mediating SigT degradation during differentiation of Streptomyces coelicolor
Author(s) -
Mao Xu-Ming,
Ren Ni-Ni,
Sun Ning,
Wang Feng,
Zhou Ri-Cheng,
Tang Yi,
Li Yong-Quan
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.12.029
Subject(s) - proteasome , streptomyces coelicolor , actinorhodin , degradation (telecommunications) , microbiology and biotechnology , biology , protease , degron , protein degradation , biochemistry , mutant , ubiquitin , gene , enzyme , ubiquitin ligase , telecommunications , computer science
In Streptomyces coelicolor , the ECF sigma factor SigT negatively regulates cell differentiation, and is degraded by ClpP protease in a dual positive feedback manner. Here we further report that the proteasome is required for degradation of SigT, but not for degradation of its anti‐sigma factor RstA, and RstA can protect SigT from degradation independent of the proteasome. Meanwhile, deletion of the proteasome showed reduced production of secondary metabolites, and the fermentation medium from wild type could promote SigT degradation. Furthermore, overexpression of redD or actII‐orf4 in the proteasome‐deficiency mutant resulted in SigT degradation and over‐production of both undecylprodigiosin and actinorhodin. Therefore the proteasome is required for SigT degradation by affecting the production of secondary metabolites during cell differentiation.