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2,3‐Dihydroxy‐quinoxaline induces ATPase activity of Herpes Simplex Virus thymidine kinase
Author(s) -
Zeifman Alexey A.,
Novikov Fedor N.,
Stroylov Victor S.,
Stroganov Oleg V.,
Chilov Ghermes G.,
Skoblov Alexander Y.,
Miroshnikov Anatoly I.,
Skoblov Yuri S.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.12.017
Subject(s) - quinoxaline , thymidine kinase , herpes simplex virus , chemistry , kinase , phosphorylation , enzyme , virology , hydrolysis , thymidine , ic50 , microbiology and biotechnology , virus , biochemistry , biology , in vitro , organic chemistry
2,3‐Dihydroxy‐quinoxaline, a small molecule that promotes ATPase catalytic activity of Herpes Simplex Virus thymidine kinase (HSV‐TK), was identified by virtual screening. This compound competitively inhibited HSV‐TK catalyzed phosphorylation of acyclovir with K i = 250 μM (95% CI: 106–405 μM) and dose‐dependently increased the rate of the ATP hydrolysis with K M = 112 μM (95% CI: 28–195 μM). The kinetic scheme consistent with this experimental data is proposed.