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Fructose promotes the differentiation of 3T3‐L1 adipocytes and accelerates lipid metabolism
Author(s) -
Legeza Balázs,
Balázs Zoltán,
Odermatt Alex
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.12.014
Subject(s) - fructose , lipolysis , lipid droplet , chemistry , endocrinology , medicine , endoplasmic reticulum , 3t3 l1 , adipocyte , glycolysis , carbohydrate metabolism , adipose tissue , peroxisome , lipid metabolism , dehydrogenase , adipogenesis , metabolism , biochemistry , biology , enzyme , receptor
Excessive fructose consumption and elevated glucocorticoids contribute to metabolic syndrome. We show that fructose as the only carbohydrate source is sufficient for the differentiation of 3T3‐L1 fibroblasts into adipocytes. Differentiation of cells in fructose containing medium resulted in increased 11β‐hydroxysteroid dehydrogenase 1 (11β‐HSD1) expression and activity. Experiments with transfected HEK‐293 cells suggested more efficient NADPH generation by fructose compared with glucose in the endoplasmic reticulum (ER). Adipocytes differentiated in the presence of fructose showed increased FABP4 expression, C/EBPα to C/EBPβ ratio and lipolysis. Thus, excessive fructose may cause adverse metabolic effects by enhancing 11β‐HSD1 activity and increasing lipolysis in adipocytes.