Premium
Rre37 stimulates accumulation of 2‐oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803
Author(s) -
Joseph Ancy,
Aikawa Shimpei,
Sasaki Kengo,
Teramura Hiroshi,
Hasunuma Tomohisa,
Matsuda Fumio,
Osanai Takashi,
Hirai Masami Yokota,
Kondo Akihiko
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.12.008
Subject(s) - citric acid cycle , biochemistry , glycogen , glycolysis , catabolism , fructose , sugar phosphates , glycogen synthase , anabolism , biology , chemistry , metabolism , phosphate
Rre37 (sll1330) in a cyanobacterium Synechocystis sp. PCC 6803 acts as a regulatory protein for sugar catabolic genes during nitrogen starvation. Low glycogen accumulation in Δ rre37 was due to low expression of glycogen anabolic genes. In addition to low 2‐oxoglutarate accumulation, normal upregulated expression of genes encoding glutamate synthases ( gltD and gltB ) as well as accumulation of metabolites in glycolysis (fructose‐6‐phosphate, fructose‐1,6‐bisphosphate, and glyceraldehyde‐3‐phosphate) and tricarboxylic acid (TCA) cycle (oxaloacetate, fumarate, succinate, and aconitate) were abolished by rre37 knockout. Rre37 regulates 2‐oxoglutarate accumulation, glycogen accumulation through expression of glycogen anabolic genes, and TCA cycle metabolites accumulation.