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MicroRNA‐344 inhibits 3T3‐L1 cell differentiation via targeting GSK3β of Wnt/β‐catenin signaling pathway
Author(s) -
Chen Hu,
Wang Siqi,
Chen Luxi,
Chen Yaosheng,
Wu Ming,
Zhang Yun,
Yu Kaifan,
Huang Zheng,
Qin Lijun,
Mo Delin
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.12.002
Subject(s) - wnt signaling pathway , gsk 3 , microbiology and biotechnology , microrna , signal transduction , gene knockdown , gsk3b , 3t3 l1 , cellular differentiation , 3t3 cells , biology , effector , chemistry , cell culture , transfection , biochemistry , genetics , adipogenesis , mesenchymal stem cell , gene
Differentiation of 3T3‐L1 cells into adipocytes involves a highly orchestrated series of complex events in which microRNAs might play an essential role. In this study, we found that the overexpression of microRNA‐344 (miR‐344) inhibits 3T3‐L1 cell differentiation and decreases triglyceride accumulation after MDI stimulation. We demonstrated that miR‐344 directly targets the 3′ UTR of GSK3β (Glycogen synthase kinase 3 beta). Knockdown of GSK3β with siRNA results in inhibiting 3T3‐L1 differentiation, while its overexpression restores the effect of miR‐344. In addition, miR‐344 elevates the level of active β‐catenin, which is the downstream effector of GSK3β in the Wnt/β‐catenin signaling pathway. These data indicate that miR‐344 inhibits adipocyte differentiation via targeting GSK3β and subsequently activating the Wnt/β‐catenin signaling pathway.