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Sphingolipid levels crucially modulate lateral microdomain organization of plasma membrane in living yeast
Author(s) -
Vecer Jaroslav,
Vesela Petra,
Malinsky Jan,
Herman Petr
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.11.038
Subject(s) - sphingolipid , lipid microdomain , ceramide , microbiology and biotechnology , membrane , saccharomyces cerevisiae , yeast , chemistry , biochemistry , biophysics , biology , apoptosis
We report sphingolipid‐related reorganization of gel‐like microdomains in the plasma membrane of living S accharomyces cerevisiae using trans ‐Parinaric acid (t‐PnA) and 1,6‐diphenyl‐1,3,5‐hexatriene (DPH). Compared to control, the gel‐like domains were significantly reduced in the membrane of a sphingolipid‐deficient lcb 1 ‐ 100 mutant. The same reduction resulted from sphingolipid depletion by myriocin. The phenotype could be reverted when a myriocin‐induced block in sphingolipid biosynthesis was bypassed by exogenous dihydrosphingosine. Lipid order of less‐ordered membrane regions decreased with sphingolipid depletion as well, as documented by DPH fluorescence anisotropy. The data indicate that organization of lateral microdomains is an essential physiological role of these structural lipids.