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Effects of ribosomes on the kinetics of Qβ replication
Author(s) -
Usui Kimihito,
Ichihashi Norikazu,
Kazuta Yasuaki,
Matsuura Tomoaki,
Yomo Tetsuya
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.11.018
Subject(s) - ribosome , rna , replication (statistics) , rna dependent rna polymerase , translation (biology) , replication factor c , kinetics , microbiology and biotechnology , biology , viral replication , biophysics , chemistry , computational biology , dna replication , genetics , physics , messenger rna , dna , control of chromosome duplication , virology , virus , gene , quantum mechanics
Bacteriophage Qβ utilizes some host cell translation factors during replication. Previously, we constructed a kinetic model that explains replication of long RNA molecules by Qβ replicase. Here, we expanded the previous kinetic model to include the effects of ribosome concentration on RNA replication. The expanded model quantitatively explained single‐ and double‐strand formation kinetics during replication with various ribosome concentrations for two artificial long RNAs. This expanded model and the knowledge obtained in this study provide useful frameworks to understand the precise replication mechanism of Qβ replicase with ribosomes and to design amplifiable RNA genomes in translation‐coupling systems.