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N‐terminal signal sequence is required for cellular trafficking and hyaluronan‐depolymerization of KIAA1199
Author(s) -
Yoshida Hiroyuki,
Nagaoka Aya,
Nakamura Sachiko,
Tobiishi Megumi,
Sugiyama Yoshinori,
Inoue Shintaro
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.11.017
Subject(s) - depolymerization , endosome , microbiology and biotechnology , endocytosis , chemistry , vesicle , cleavage (geology) , intracellular , biophysics , biochemistry , biology , cell , membrane , paleontology , organic chemistry , fracture (geology)
Recently, we disclosed that KIAA1199‐mediated hyaluronan (HA) depolymerization requires an acidic cellular microenvironment (e.g. clathrin‐coated vesicles or early endosomes), but no information about the structural basis underlying the cellular targeting and functional modification of KIAA1199 was available. Here, we show that the cleavage of N‐terminal 30 amino acids occurs in functionally matured KIAA1199, and the deletion of the N‐terminal portion results in altered intracellular trafficking of the molecule and loss of cellular HA depolymerization. These results suggest that the N‐terminal portion of KIAA1199 functions as a cleavable signal sequence required for proper KIAA1199 translocation and KIAA1199‐mediated HA depolymerization.