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Expression, phosphorylation and function of the Rab‐GTPase activating protein TBC1D1 in pancreatic beta‐cells
Author(s) -
Rütti Sabine,
Arous Caroline,
Nica Alexandra C.,
Kanzaki Makoto,
Halban Philippe A.,
Bouzakri Karim
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.10.050
Subject(s) - rab , medicine , microbiology and biotechnology , gtpase activating protein , endocrinology , protein kinase b , beta cell , beta (programming language) , biology , phosphorylation , gtpase , insulin , chemistry , signal transduction , g protein , islet , computer science , programming language
The Rab‐GTPase activating protein TBC1D1 is a paralog of AS160/TBC1D4. AS160/TBC1D4, a downstream effector of Akt, has been shown to play a central role in beta‐cell function and survival. The two proteins have overlapping function in insulin signalling in muscle cells. However, the expression and the potential role of TBC1D1 in beta‐cells remain unknown. Therefore, the aim of this study is to investigate whether TBC1D1 is expressed in beta‐cells and whether it plays, as AS160/TBC1D4, a role in beta‐cell function and survival. Using human and rat beta‐cells, this study shows for the first time that TBC1D1 is expressed and phosphorylated in response to glucose in these cells. Knockdown of TBC1D1 in beta‐cells resulted in increased basal and glucose‐stimulated insulin release, decreased proliferation but no change in apoptosis.