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ARP101 inhibits α‐MSH‐stimulated melanogenesis by regulation of autophagy in melanocytes
Author(s) -
Kim Eun Sung,
Jo Yoon Kyung,
Park So Jung,
Chang Huikyoung,
Shin Ji Hyun,
Choi Eun Sun,
Kim Jun Bum,
Seok Su Hyeon,
Kim Jae-Sung,
Oh Jeong Su,
Kim Myoung-Hwan,
Lee Eunjoo H.,
Cho Dong-Hyung
Publication year - 2013
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.10.027
Subject(s) - autophagy , melanosome , atg5 , microbiology and biotechnology , melanin , tyrosinase , microphthalmia associated transcription factor , melanocyte , chemistry , organelle , gene knockdown , biology , apoptosis , melanoma , biochemistry , cancer research , enzyme
Autophagy is a cooperative process between autophagosomes and lysosomes that degrades cellular organelles. Although autophagy regulates the turnover of cellular components, its role in melanogenesis is not clearly established. Previously, we reported that ARP101 induces autophagy in various cancer cells. Here, we show that ARP101 inhibits melanogenesis by regulation of autophagy. ARP101 inhibited α‐MSH‐stimulated melanin synthesis and suppressed the expression of tyrosinase and TRP1 in immortalized mouse melanocytes. ARP101 also induced autophagy in melanocytes. Knockdown of ATG5 reduced both anti‐melanogenic activity and autophagy mediated by ARP101 in α‐MSH treated melanocytes. Electron microscopy analysis further revealed that autophagosomes engulf melanin or melanosome in α‐MSH and ARP101‐treated cells. Collectively, our results suggest that ARP101 inhibits α‐MSH‐stimulated melanogenesis through the activation of autophagy in melanocytes.