Premium
Engine out of the chassis: Cell‐free protein synthesis and its uses
Author(s) -
Rosenblum Gabriel,
Cooperman Barry S.
Publication year - 2014
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2013.10.016
Subject(s) - protein biosynthesis , translation (biology) , cell free protein synthesis , chassis , lysis , microbiology and biotechnology , protein expression , synthetic biology , in vivo , cell free system , cell , computer science , chemistry , computational biology , biochemistry , in vitro , biology , messenger rna , engineering , gene , structural engineering
The translation machinery is the engine of life. Extracting the cytoplasmic milieu from a cell affords a lysate capable of producing proteins in concentrations reaching to tens of micromolar. Such lysates, derivable from a variety of cells, allow the facile addition and subtraction of components that are directly or indirectly related to the translation machinery and/or the over‐expressed protein. The flexible nature of such cell‐free expression systems, when coupled with high throughput monitoring, can be especially suitable for protein engineering studies, allowing one to bypass multiple steps typically required using conventional in vivo protein expression.